解剖学报

›› 2011, Vol. 42 ›› Issue (3): 384-388.doi: 10.3969/j.issn.0529-1356.2011.03.018

• 论著 • 上一篇    下一篇

蛋白磷酸酶-2A催化亚基C在小鼠不同器官中的分化表达与定位

刘文彬1;惠珊珊1 ;胡小慧1 ;聂磊1;马慧1 ;王玲1;肖亚梅1 ;李万程1, 2,3*   

  1. 1.湖南师范大学生命科学学院，蛋白质化学与发育生物学教育部重点实验室，长沙 410081; 2.美国内布拉斯加州大学医学院生物化学与分子生物学系，Nebraska Omaha 68198;3.美国内布拉斯加州大学医学院眼科研究中心， Nebraska Omaha 68198
  • 收稿日期:2011-01-19 修回日期:2011-03-02 出版日期:2011-06-06
  • 通讯作者: 李万程

Differential expression and distribution of the catalytic subunit C for protein phosphatase-2A in the different organs of mouse

  1. 1. Key Laboratory of Protein Chemistry and Development Biology of National Education Ministry, College of Life Science, Hunan Normal University, Changsha 410081, China; 2. Department of Biochemistry and Molecular Biology, Ophthalmology & Visual Sciences, Nebraska 68198,USA;3.Department of College of Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198,USA
  • Received:2011-01-19 Revised:2011-03-02 Online:2011-06-06
  • Contact: LI Wan-cheng

关键词: 蛋白质去磷酸化, 蛋白磷酸酶-2A, 催化亚基, 信号转导, 反转录-聚合酶链式反应, 免疫印迹法, 免疫荧光, 小鼠

Abstract: Objective Protein serine/threonine phosphatase-2A (PP-2A) is one of the most important phosphatases in eukaryotes. To lay down a basis for exploring its physiological functions in mammals, we have analyzed the expression patterns and distribution of the catalytic subunits for PP-2A in the different organs of 6 adult mice. Methods RT-PCR，Western blotting analysis and immunofluorescence histochemistry were used to establish the expression patterns and distribution in various tissues of the adult mouse. Results RT-PCR revealed that the PP-2Ac mRNAs were expressed at the highest levels in the brain, at reduced levels in the ovary, at much reduced levels in the muscle, kidney, spleen, heart, liver, lung and breast, at the lowest levels in the stomach. Western blotting analysis demonstrated that the highest protein expression level of the PP-2Ac proteins was also detected in the brain. A reduced level of this protein was found in the ovary, spleen, heart, liver and lung. A much reduced PP-2Ac protein expression level was detected in the muscle, kidney and breast. And the lowest expression level of PP-2Ac was detected in the stomach.Immunohistochemistry analysis revealed the specificity of PP-2Ac distribution at both cellular levels and tissue levels. Conclusion Our results suggest that PP-2A is one of the most important protein phosphatases in mammalians.

Key words: Dephosphorylation, Protein phosphatase-2A, Catalytic subunit, Signal transduction, RT-PCR, Western blotting, Immunofluorescence, Mouse

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